Received: 10.03.2023 / Accepted: 19.05.2023 / Published online: 30.06.2023
УДК 615.07
DOI 10.53511/PHARMKAZ.2023.38.14.040
Zhurynov M.Zh.1, Miftakhova A.F.1.2, Khalykberbiyev M.K.1, Solodova E.V.1,2, Murat A.2, Ryskulbekova A.2
1AO «D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry. Almaty, Republic of Kazakhstan
2Al-Farabi Kazakh National University, Almaty, Republic of Kazakhstan
OBTAINING DRY EXTRACTS FROM LICORICE ROOTS (GLYCYRRHIZA GLABRA)
AND ITS STANDARDIZATION
Resume. Licorice (Glycyrrhiza glabra) root has long been used in traditional medicine due to its various
therapeutic properties. One of the main active components in licorice root is glycyrrhizin, which has been
shown to possess numerous pharmacological effects. Glycyrrhizin, one of the major compounds present in
licorice root, has been shown to possess anti-inflammatory, antioxidant, and anti-tumor propertiesas well as
antiviral properties against influenza virus and coronavirus COVID-19. The demand for licorice root extracts is
increasing, particularly for use in the food and pharmaceutical industries. However, the quality and consistency
of licorice extracts can vary depending on the extraction method used. Therefore, the aim of this study was
to optimise the technology for obtaining dry extracts from licorice roots and standardise the process for
increased consistency and quality of the final product. In this study, two different methods for extraction were
compared: ultrasound-assisted extraction using water and alcohol-water solvents. The extracted samples were
then subjected to various quality control tests, including glycyrrhizin content and moisture content. The content
of glycyrrhizin in the obtained dry extracts was determined using high-performance liquid chromatography
(HPLC). The standardisation of the dry extract was performed according to the Pharmacopoeia of the
Eurasian Economic Union. The results showed that the optimised technology for obtaining dry extracts from
licorice roots was water extraction with ultrasound, as it yielded a higher content of glycyrrhizin compared
to ethanol extraction with ultrasoundand the optimal conditions for ultrasound-assisted extraction were an
extraction time of 60 minutes, frequency of ultrasound bath at 40 kHz, an extraction temperature of 30°C,
and a solid-to-liquid ratio of 1:6. The standardisation process confirmed the presence of glycyrrhizic acid
and purity of the obtained dry extract.
Key words: licorice root,glycyrrhizic acid, glycyrrhizaglabra, ultrasound, extraction, standardisation, solvent.
REFERENCES
1 Deutch R., Grimm M., Wehland D., Infanger M., Krüger M. Bioactive Candy: Effects of Licorice on the Cardiovascular System. Foods. 2019;495(8): 2-12.
2 Kuriyama A., Maeda, H. Topical application of licorice for prevention of postoperative sore throat in adults: A systematic review and meta-analysis. Journal
of Clinical Anesthesia. 2019;54(25-32):1-2.
3 Huan C., Xu, ZhangY.,GuoW., PanT., Gao S. Research Progress on the Antiviral Activity of Glycyrrhizin and its Derivatives in Liquorice. Front Pharmacol.
2021;12(680674): 1-6.
4 Kumar K., Srivastav S., Singh Sharanagat V. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products:
A review. Ultrason Sonochem. 2021;70(105325):1-6.
5 Фармакопея Союза. Евразийская экономическая комиссия. https://eec.eaeunion.org/comission/department/deptexreg/formirovanie-obshchikh-rynkov/
pharmacopoeia/ (Дата обращения 20.02.2023) [Farmakopeya Soyuza. Evrazijskayaeko nomicheskaya komissiya. https://eec.eaeunion.org/comission/
department/deptexreg/formirovanie-obshchikh-rynkov/pharmacopoeia/ (Data obrashcheniya 20.02.2023)]
6 ОФС.1.2.1.0010.15 Потеря в массе при высушивании. http://pharmacopoeia.ru/ofs-1-2-1-0010-15-poterya-v-masse-pri-vysushivanii/ (Дата обращения
20.02.2023) [OFS.1.2.1.0010.15 Poterya v masse pri vysushivanii. http://pharmacopoeia.ru/ofs-1-2-1-0010-15-poterya-v-masse-pri-vysushivanii/ (Data
obrashcheniya 20.02.2023)]
7 Wang, B., Luo, X., Wang, J., Jin, Y., Wang, T., Yu, X., & Yin, G. Study on the Preparation and Characterization of Glycyrrhizic Acid Liposomes. Scientific
Research Publishing. 2022;13:206.
