VERIFICATION OF ANALYTICAL METHODS IN THE ANALYSIS OF RHODIOLA HETRODONTHA (HOOK. F. ET THOMSON) BORISS

Алынды: 24.10.2024/ Қабылданды: 19.11.2024/ Онлайн жарияланды: 30.12.2024
UDC 543.9:582.683.2
DOI: 10.53511/pharmkaz.2025.95.68.037
S.S. Dzhaborova 1, L.N. Ibragimova 3, Z.B. Allambergenova 3, S.J. Yusufi 2, M.B. Ibrayeva 3, M. Iman 4, Z.B. Sakipova 3
1 Abuali ibni Sino Tajik State Medical University, Dushanbe, the Republic of Tajikistan
2 Ministry of Health and Social Protection of the Population of Tajikistan Dushanbe, the Republic of Tajikistan
3 S.D. Asfendiyarov Kazakh National Medical University, Almaty, the Republic of Kazakhstan
4 Haileybury School, Almaty, the Republic of Kazakhstan
VERIFICATION OF ANALYTICAL METHODS IN THE ANALYSIS OF RHODIOLA HETRODONTHA (HOOK. F. ET THOMSON)
BORISS
Resume. The article presents the verification of pharmacopoeial identification and quantitative determination methods as
applied to the raw material of Rhodiola hetrodontha. Characteristic of the genus Rhodiola SPP. plant phenolic compounds such
as salidroside and tyrosol were determined in the rhizomes and the roots of Rhodiola hetrodontha, and rosarin was not
identified. The verification of the pharmacopoeial method for the quantitative determination of genus-specific phenolic
compounds in the rhizomes and roots of Rhodiola hetrodontha was carried out, which consists of the extraction of phenolic
compounds with 70% ethyl alcohol and analysis by high-performance liquid chromatography.
The optimality of the pharmacopoeial conditions for chromatographic analysis (HPLC and FETLC) of genus-specific phenolic
compounds in the rhizomes and roots of Rhodiola hetrodonta has been confirmed.
The specificity, linearity, correctness, accuracy and range of application of the studied methods have been proven. Therefore
they can be used in quality control of the rhizomes and roots of Rhodiola hetrodonta.
Key words. Verification, analytical method, identification, quantitative determination, pharmacopoeia, medicinal plant
materials, Rhodiola hetrodontha (Hook. f. et Thomson) Boriss

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