Received: 22 July 2026
Accepted: 09 October 2026
Published online: 30 October 2026
UDC: 582.6/.9
DOI: 10.53511/pharmkaz.2026.5.15
Article type: Original Research Article
IDENTIFICATION AND QUANTITATIVE DETERMINATION OF FLAVONOIDS IN DODARTIA ORIENTALIS HERB
MukhidinZh.N. 1, Orynbasarova K.K. 2, RakhmanovaG.S. 2, TursubekovaB.I. 1
1M. Auezov South Kazakhstan Research University, Shymkent, Republic of Kazakhstan
2JSC «South Kazakhstan Medical Academy», Shymkent, Republic of Kazakhstan
Introduction. The article is devoted to the urgent issue of developing effective phytopreparations based on accessible plant raw materials for domestic pharmaceutical science and industry. According to literature data, the plant is rich in biologically active compounds and exhibits anti-inflammatory, antimicrobial, and anti-allergic activities. However, the chemical composition and flavonoid profile of this plant, which is widespread in the local flora, still require in-depth study from the perspective of modern pharmacy. Given the development of phytotherapy, a systematic analysis of the chemical composition of Dodartia orientalis is of great importance. Furthermore, these results serve as a scientific and practical basis for the development, standardization, and further evaluation of the pharmacological potential of highly effective and safe domestic phytopreparations and liquid extracts derived from this herbal raw material.
Aim. Select an extracting agent with an optimal concentration for isolating flavonoids from the aerial parts of Dodartia orientalis L. and using the result extract for IR spectroscopy and HPLC methods for analyzing flavonoids.
Methods. In the course of the study, ethanol solutions of various concentrations were used, and the suitability of 70% ethanol as an extractant for the isolation of flavonoids was evaluated. Quantitative evaluation of the total flavonoid content in the extracts was performed using IR spectroscopy and HPLC methods in accordance with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan.
Results. FT-IR spectroscopy revealed the presence of functional groups characteristic of flavonoids in the extract of Dodartia orientalis. Hyperoside (0,963%), rutin (0,412%), and dihydroquercetin (0,341%) were identified using HPLC. The total content of the three flavonoids was 1,716% based on absolutely dry raw material.
Discussion. IR-spectroscopy revealed the presence of functional groups characteristic of flavonoids in the extract of Dodartia orientalis and showed identity to the hyperoside reference standard. The main phenolic compounds with high antioxidant activity – rutin, hyperosid, and dihydroquercetin – were identified in the plant material.
Conclusion. The results obtained serve as a foundation for expanding the raw material base of the medicinal herb Dodartia orientalis.
Keywords: Dodartia orientalis, chemical analysis, extraction, flavonoids, IR-spectroscopy, biologically active substances, State Pharmacopoeia, HPLC.
