Received: 19 May 2026
Accepted: 26 September 2026
Published online: 30 October 2026
UDC: 615.322:543.544.5:615.07
DOI: 10.53511/pharmkaz.2026.5.17
Article type: Original Research Article
(Experimental Study)
DEVELOPMENT OF A METHOD FOR THE QUALİTY CONTROL OF ALTHAEA OFFICINALIS L. ROOT EXTRACT
Suleymanov T.A.¹, Balaeva E.Z.¹, Farajova J.A.¹
¹ Azerbaijan Medical University, Department of Pharmaceutical Chemistry, Baku, Azerbaijan
Introduction and Objective. Carbohydrate profiling is relevant to the quality assessment of Althaea officinalis L. root extract. This study aimed to develop HPLC conditions with refractive index detection (RID) for glucose and fructose determination in the extract and to evaluate selected analytical performance characteristics.
Materials and Methods. Powdered Althaea officinalis root extract and glucose and fructose reference standards were analyzed using an Agilent 1260 Infinity II HPLC–RID system. Retention-time and peak-area repeatability, system suitability, and limits of detection (LOD) and quantification (LOQ) were assessed.
Results. The chromatographic conditions were an injection volume of 10 µL, a column temperature of 40 °C, a flow rate of 1.0 mL/min, and an acetonitrile–water mobile phase (750:250, v/v). Peak-area RSDs for reference solutions were 0.476% for glucose and 0.789% for fructose. LOD/LOQ values were 0.059/0.180 mg/mL for glucose and 0.073/0.221 mg/mL for fructose.
Discussion and Conclusions. The results indicate acceptable injection repeatability for the two sugars under the reported conditions. They do not demonstrate direct quantification of intact polysaccharides. Further evaluation of selectivity, accuracy, calibration range and robustness is required before the procedure can be considered fully validated for routine quality control.
Keywords: Althaea officinalis L.; root extract; glucose; fructose; HPLC-RID; system suitability; quality control.
